Four-Dimensional Monitoring of Tumour Growth by Surface Enhanced Raman Scattering
ERC Advanced Grant Project
4DbioSERS is an ERC Advanced Grant Project granted to Prof. Luis Liz-Marzán by the European Research Council under the European's Union Horizon 2020 (H2020-EU.1.1) /ERC grant agreement n° 787510. The 5 year research project started the 1st of October of 2018 and will run until the 30th of September 2023. More details.
Summary
Optical bioimaging is limited by visible light penetration depth and
stability of fluorescent dyes over extended periods of time. Surface
enhanced Raman scattering (SERS) offers the possibility to overcome
these drawbacks, through SERS-encoded nanoparticle tags, which can
be excited with near-IR light (within the biological transparency
window), providing high intensity, stable, multiplexed signals. SERS
can also be used to monitor relevant bioanalytes within cells and
tissues, during the development of diseases, such as tumours. In
4DBIOSERS we shall combine both capabilities of SERS, to go well
beyond the current state of the art, by building three-dimensional
scaffolds that support tissue (tumour) growth within a controlled
environment, so that not only the fate of each (SERS-labelled) cell
within the tumour can be monitored in real time (thus adding a
fourth dimension to SERS bioimaging), but also recording the release
of tumour metabolites and other indicators of cellular activity.
Although 4DBIOSERS can be applied to a variety of diseases, we shall
focus on cancer, melanoma and breast cancer in particular, as these
are readily accessible by optical methods. We aim at acquiring a
better understanding of tumour growth and dynamics, while avoiding
animal experimentation. 3D printing will be used to generate hybrid
scaffolds where tumour and healthy cells will be co-incubated to
simulate a more realistic environment, thus going well beyond the
potential of 2D cell cultures. Each cell type will be encoded with
ultra-bright SERS tags, so that real-time monitoring can be achieved
by confocal SERS microscopy. Tumour development will be correlated
with simultaneous detection of various cancer biomarkers, during
standard conditions and upon addition of selected drugs. The scope
of 4DBIOSERS is multidisciplinary, as it involves the design of
high-end nanocomposites, development of 3D cell culture models and
optimization of emerging SERS tomography methods.
Publications
 3. |
J. Plou, M. Charconnet, I. García, J. Calvo, L.M.
Liz-Marzán, Preventing Memory Effects in
Surface-Enhanced Raman Scattering Substrates by Polymer
Coating and Laser-Activated Deprotection ACS Nano 2021, 15, 8984-8995 |
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| 2. |
C. Sanchez-Cano, R. Alvarez-Puebla, J.M. Abendroth, T.
Beck, R. Blick, Y. Cao, F. Caruso, I. Chakraborty, H.
Chapman, C. Chen, B.E. Cohen, A.L.C. Conceição, D.P.
Cormode, D. Cui, K.A. Dawson, G. Falkenberg, C. Fan, N.
Feliu, M. Gao, E. Gargioni, C.-C. Glüer, F. Grüner, M.
Hassan, Y. Hu, Y. Huang, S. Huber, N. Huse, Y. Kang, A.
Khademhosseini, T. Keller, C. Körnig, N.A. Kotov, D. Koziej,
X.-J. Liang, B. Liu, S. Liu, Y. Liu, Z. Liu, L.M.
Liz-Marzán, X. Ma, A. Machicote, W. Maison, A. Mancuso, S.
Megahed, B. Nickel, F. Otto, C. Palencia-Ramirez, S.
Pascarelli, A. Pearson, O. Peñate-Medina, B. Qi, J. Rädler,
J.J. Richardson, A. Rosenhahn, K. Rothkamm, M. Rübhausen,
M.K. Sanyal, R.E. Schaak, H.-P. Schlemmer, M. Schmidt, O.
Schmutzler, T. Schotten, F. Schulz, A.K. Sood, K.M. Spiers,
T. Staufer, D. Stemer, A. Stierle, X. Sun, G. Tsakanova, T.
Tschentscher, P.S. Weiss, H. Weller, F. Westermeier, M. Xu,
H. Yan, Y. Zeng, Y. Zhao, Y. Zhao, D. Zhu, Y. Zhu, W.J.
Parak, X-Ray-Based Techniques to Study the Nano-Bio
Interface ACS Nano 2021, 15, 3754-3807 |
| 1. |
E. Er, A. Sánchez-Iglesias, A. Silvestri, B. Arnaiz, L.M.
Liz-Marzán, M. Prato, A. Criado, Metal
nanoparticles/MoS2 SERS-Based Sandwich Immunoassay for
alpha-Fetoprotein Detection ACS Appl. Mater. Interfaces 2021, 13, 8823-8831 |
| 14. | Y. Zhang, D. Jimenez de Aberasturi, M. Henriksen-Lacey, J.
Langer, L.M. Liz-Marzán, Live Cell Surface-Enhanced
Raman Spectroscopy Imaging of Intracellular pH: From Two
Dimensions to Three Dimensions ACS Sens. 2020, 5, 3194-3206 |
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| 13. |
S. Rodal-Cedeira, A. Vázquez-Arias,
G. Bodelón, A. Skorikov, S. Nuñez-Sánchez, A. La Porta, L.
Polavarapu, S. Bals, L.M. Liz-Marzán, J. Pérez-Juste, I.
Pastoriza-Santos, An Expanded Surface-Enhanced Raman
Scattering Tags Library by Combinatorial Encapsulation of
Reporter Molecules in Metal Nanoshells ACS Nano 2020, 14, 14655-14664 |
| 12. | A. Sánchez-Iglesias, X. Zhuo, W.
Albrecht, S. Bals, L.M. Liz-Marzán, Tuning Size and
Seed Position in Small Silver Nanorods ACS Mater. Lett. 2020, 2, 1246-1250 |
| 11. | J.E.L. Villa, I. García, D.Jimenez de Aberasturi, V.
Pavlov, M.P.T. Sotomayor, L.M. Liz-Marzán, SERS-Based
Immunoassay for Monitoring Cortisol-Related Disorders Biosens. Bioelectron. 2020, 165, 112418. Free to read until September 10 |
| 10. |
L.H.G. Tizei, V. Mkhitaryan, H. Lourenço-Martins, L.
Scarabelli, K. Watanabe, T. Taniguchi, M. Tencé, J.-D.
Blazit, X. Li, A. Gloter, A. Zobelli, Luis M. Liz-Marzán,
F.J. Garcia de Abajo, O. Stéphan, M. Kociak, Tailored
Nanoscale Plasmon-Enhanced Vibrational Electron
Spectroscopy Nano Lett. 2020, 20, 2973-2979 |
| 9. | C. Vila-Parrondo, C. García-Astrain, L.M. Liz-Marzán, Colloidal
Systems Toward 3D Cell Culture Scaffolds Adv. Coll. Interface Sci. 2020, 283, 102237. Free to read until October 8 |
| 8. | C. García-Astrain, E. Lenzi, D.
Jimenez de Aberasturi, M. Henriksen-Lacey, M. Binelli, L.M.
Liz-Marzán, 3D-Printed Biocompatible Scaffolds with
Built-In Nanoplasmonic Sensors Adv. Funct. Mater. 2020, 30, 2005407 |
| 7.
|
X. Zhuo, M. Henriksen-Lacey, D. Jimenez de Aberasturi, A.
Sánchez-Iglesias, L.M. Liz-Marzán, Shielded Silver
Nanorods for Bioapplications Chem. Mater. 2020, 32, 5879-5889 |
| 6. | G. González-Rubio, J. Mosquera, V.
Kumar, A. Pedrazo-Tardajos, P. Llombart, D.M. Solís, I.
Lobato, E.G. Noya, A. Guerrero-Martínez, J.M. Taboada, F.
Obelleiro, L.G. MacDowell, S. Bals, L.M. Liz-Marzán, Micelle-Directed
Chiral Seeded Growth on Anisotropic Gold Nanocrystals Science 2020, 368, 1472-1477. See REPRINT |
| 5. |
J. Mosquera, I. García, M. Henriksen-Lacey, M.
Martínez-Calvo, M. Dhanjani, J.L. Mascareñas, L.M.
Liz-Marzán, Reversible Control of Protein Corona
Formation on Gold Nanoparticles Using Host-Guest
Interactions ACS Nano 2020, 14, 5382-5391 |
| 4. |
J. Plou, I. García, M. Charconnet, I. Astobiza, C.
García-Astrain, C. Matricardi, A. Mihi, A. Carracedo, L.M.
Liz-Marzán, Multiplex SERS Detection of Metabolic
Alterations in Tumor Extracellular Media Adv. Funct. Mater. 2020, 30, 1910335 |
| 3. |
D. Jimenez de Aberasturi, M. Henriksen-Lacey, L. Litti, J.
Langer, L.M. Liz-Marzán, Using SERS Tags to Image
the Three-Dimensional Structure of Complex Cell Models Adv. Funct. Mater. 2020, 30, 1909655 |
| 2. |
L. Litti, J. Reguera, F.J. García de Abajo, M. Meneghetti,
L.M. Liz-Marzán, Manipulating Chemistry through
Nanoparticle Morphology Nanoscale Horiz. 2020, 5, 102-108 |
| 1. |
J. Langer, D. Jimenez de Aberasturi, J. Aizpurua, R.A.
Alvarez-Puebla, B. Auguié, J.J. Baumberg, G.C. Bazan, S.E.
Bell, A. Boisen, A. Brolo, J. Choo, D. Cialla-May, V.
Deckert, L. Fabris, K. Faulds, F.J. García de Abajo, R.
Goodacre, D. Graham, A.J. Haes, C.L. Haynes, C. Huck, T.
Itoh, M. Käll, J. Kneipp, N.A. Kotov, H. Kuang, E.C. Le Ru,
H.K. Lee, J.-F. Li, X.Y. Ling, S.A. Maier, T. Mayerhöfer, M.
Moskovits, K. Murakoshi, J.-M. Nam, S. Nie, Y. Ozaki, I.
Pastoriza-Santos, J. Pérez-Juste, J. Popp, A. Pucci, S.
Reich, B. Ren, G.C. Schatz, T. Shegai, S. Schlücker, L.-L.
Tay, K.G. Thomas, Z.-Q. Tian, R.P. Van Duyne, T. Vo-Dinh, Y.
Wang, K.A. Willets, C. Xu, H.X. Xu, Y. Xu, Y.S. Yamamoto, B.
Zhao, L.M. Liz-Marzán, Present and Future of
Surface-Enhanced Raman Scattering ACS Nano 2020, 14, 28-117 |
2019
| 4. |
M. Grzelczak, L.M. Liz-Marzán, R. Klajn, Stimuli-Responsive
Self-Assembly of Nanoparticles Chem. Soc. Rev. 2019, 48, 1342-1361 |
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| 3. |
M. Quintanilla, C. Kuttner, J.D. Smith, A. Seifert, S.E.
Skrabalak, L.M. Liz-Marzán, Heat Generation by
Branched Au/Pd Nanocrystals: Influence of Morphology and
Composition Nanoscale 2019, 11, 19561-19570 |
| 2. |
M. Quintanilla, I. García, I. de Lázaro, R. García-Alvarez,
M. Henriksen-Lacey, S. Vranic, K. Kostarelos, L.M. Liz-Marzán,
Thermal Monitoring During Photothermia: Hybrid Probes
for Simultaneous Plasmonic Heating and Near-Infrared Optical
Nanothermometry Theranostics 2019, 9, 7298-7312 |
| 1. | E. Lenzi, D. Jimenez de Aberasturi, L.M. Liz-Marzán, SERS
Tags for 3D Bioimaging and Biomarker Detection ACS Sensors 2019, 4, 1126-1137 |
Project Members
Project head: Prof. Luis Liz-Marzán
CIC biomaGUNE
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